Comparative analisis of the protective therapectic effect between embryonic tissue secretome – mixture proteins and peptides – and transplantation of neonatal kidney tissue in acute post-ishemic renal failure

68

DOI: 10.29188/2222-8543-2020-12-1-28-35

Kirpatovskiy V.I., Sivkov A.V., Golovanov S.A., Drozhzheva V.V., Frolova E.V.
№1 2020

Introduction. Regenerativemedicine is an actively developing field. The possibility oftreating chronic diseaseswith stemcelltransplantation and therapywith products of their secretion is being studied. In this study, we compared the effectiveness of therapy in rats with severe acute renal failure (ARF) using course administration of protein-peptide secretome (PPS) isolated from the brain of pig embryos and by transplanting tissue homogenate of neonatal kidneys of newborn rats.

Materials and methods. In experiments on 36white male rats,ARFwassimulated by unilateral nephrectomy and 90-minute ischemia of the remaining kidney. In the 1st series (10 rats), therapy was not performed, in the 2nd series (10 rats), immediately after restoring blood flow in the kidney PPS was subcutaneously administered at a dose of 0.1 mg/kg followed by daily administration of the drug for 10 days, and in the 3rd series (10 rats) before restoring blood supply to the kidney, a homogenate of the kidney tissue of newborn rats was injected under its capsule. The animals were examined at 3, 7, and 14 days after the ARF simulation. Six rats were a control group.

Results. PPS therapy has been shown to reduce the severity of metabolic disorders (less polyuria, increasing concentrations of creatinine and urea in the blood, decreasing glomerular filtration and reabsorption ofsodium and calcium)from 3 days.In experimentswith neonatal kidney tissue transplantation, improvement of functional parameterscompared to the control was noted only on the 7th day, but the degree of improvement was higher than in PPS therapy. The severity of fermenturia, as an indicator of the destruction of the epitheliumof the renal tubules,was also the lowest in experimentswith neonatal kidney tissue transplantation.

Conclusion. The results of the study indicate different dynamics of restoring the function of the damaged kidney in the studied variants of ARF therapy, which may be due to the peculiarities of delivering the embryonal (or neonatal) stem cells secretome to the organ (long-term through the circulatory system or once directly to the organ). The question of the significance of the organ-specific secretome for the therapeutic effect requires further study.

Conflict of interest. The authors declare no conflict of interest.

Read in the source